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Home Antibody All anti-PDK1 antibodies

Anti-PDK1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319161
  • Rabbit polyclonal anti-PDK1 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400925) , 20ug Explanation
100ug 325 3-7 Days
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WB(1)

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of human PDK-1 protein.
Clone Name IsotypeIgG
Species Reactivityhuman Concentration1.3 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:100,000, WB: 1:500 - 1:2,000, IHC: User Optimized
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note PDK-1 (3-Phosphoinositide-Dependant Protein Kinase-1) phosphorylates AGC kinases.  PDK-1 activates conventional PKC and PKCz (zeta) through phosphorylation of critical threonine residues in the activation loop. PDK-1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase-3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

Reference Data

Target NameHomo sapiens 3-phosphoinositide dependent protein kinase 1 (PDPK1), transcript variant 3
Alternative NamePDK1; PDPK2; PRO0461
Database LinkNP_001248745
Entrez Gene 5170 Human
Function
Related PathwayProtein KinaseDruggable Genome PPAR signaling pathwaymTOR signaling pathwayFocal adhesionInsulin signaling pathwayEndometrial cancerProstate cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using affinity purified anti-PDK-1 antibody shows detection of myc-tagged human PDK-1 at 60kDa in ~10 µg of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4° C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG [H&L] (p/n 611-1302) and chemiluminescent detection is recommended. Other detection systems will yield similar results.

 

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