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Also for SERPINA1 (NM_000295)
|a1-Anti-Trypsin [Human Plasma]|
||ELISA: 1:20,000 - 1:100,000, WB: 1:2,000 - 1:10,000, IHC: 1:500 - 1:2,500
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
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Goat-anti-Alpha-1-Anti-Trypsin (TA319139, red), Rabbit anti-Transferrin , and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Membrane was probed with three primary antibodies at 1:1000 dilution. Detection shown was using DyLight549 Donkey anti-Rabbit IgG, DyLight 488 Donkey anti-Mouse IgG , and DyLight 649 Donkey anti-Goat IgG.
Goat anti Alpha-1anti-Trypsin antibody was used to detect Alpha-1anti-Trypsin under reducing (R) conditions. Reduced sample of purified target protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 488 conjugated Donkey anti goat 1:10K in TBS/MB-070 1 hr RT).
Primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin ; Alpha 1 anti trypsin and Human IgG in a multiplex fluorescent WB of human serum. Each primary antibody was diluted to 1:1000 in IRdye blocking buffer (MB-070) and incubated for 2 hrs at RT. Blot was 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in IRdye blocking buffer and incubated ~ 1hr at 4 degrees.