OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-SERPINA1 antibodies

Anti-SERPINA1 Antibody

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA319139
  • Goat polyclonal Alpha 1 Anti Trypsin antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400112) , 20ug Explanation
2mL $325 3-7 Days
Add to Shopping Cart
spacer
WB(3)
spacer
Also for SERPINA1 (NM_000295)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

Immunogena1-Anti-Trypsin [Human Plasma]
Clone Name Isotype
Species Reactivityhuman Concentration90 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:20,000 - 1:100,000, WB: 1:2,000 - 1:10,000, IHC: 1:500 - 1:2,500
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Reference Data

Target NameHomo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 1
Alternative NameA1A; A1AT; AAT; alpha1AT; PI; PI1; PRO2275
Database LinkNP_000286
Entrez Gene 5265 Human
Function
Related PathwaySecreted ProteinES Cell Differentiation/IPSDruggable Genome Complement and coagulation cascades

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Goat-anti-Alpha-1-Anti-Trypsin (TA319139 lot 5842, red), Rabbit anti-Transferrin (109-4134 lot 3033), and Mouse-a-GST (200-301-200 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4°C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (611-742-127 lot 21100, shown as green)   DyLight 488 Donkey anti-Mouse IgG (610-741-124 lot 21095, shown as blue),  and DyLight 649 Donkey anti-Goat IgG (605-743-125 lot 20834, shown as red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.
spacer
Goat anti Alpha-1anti-Trypsin antibody (200-101-147 lot 5854) was used to detect Alpha-1anti-Trypsin under reducing (R) conditions. Reduced sample of purified target protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System.
spacer
primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin (1° 109-4134, green 2° 611-743-127); Alpha 1 anti trypsin (1° TA319139, red 2° 605-742-125); and Human IgG (1° 109-3102, Blue 2° 610-741-124 in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in IRdye blocking buffer (MB-070) and incubated for 2 hrs at RT. Blot was 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in IRdye blocking buffer and incubated ~ 1hr at 4 degrees. After wash 2X in TTBS and 2X in TBS, blot was rinsed 2X in MeOH, dried and imaged using the Biorad VersaDoc4000.
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title