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Home Antibody All anti-SERPINA1 antibodies

Anti-SERPINA1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319139
  • Goat polyclonal Alpha 1 Anti Trypsin antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400112) , 20ug
2mL 325 3-7 Days
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WB(3)
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Also for SERPINA1 (NM_000295)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

Immunogena1-Anti-Trypsin [Human Plasma]
Clone Name Isotype
Species Reactivityhuman Concentration90 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:20,000 - 1:100,000, WB: 1:2,000 - 1:10,000, IHC: 1:500 - 1:2,500
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Reference Data

Target NameHomo sapiens serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), transcript variant 1
Alternative NameA1A; A1AT; AAT; alpha1AT; PI; PI1; PRO2275
Database LinkNP_000286
Function
Related Pathway
Hemostasis

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WB Image
Goat-anti-Alpha-1-Anti-Trypsin (TA319139 lot 5842, red), Rabbit anti-Transferrin (109-4134 lot 3033), and Mouse-a-GST (200-301-200 lot 24882) were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Sample was run by SDS-PAGE, transferred to 0.2 um PVDF using the BioRad Trans-Blot Turbo and blocked in 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Membrane was probed with three primary antibodies at 1:1000 dilution (in MB-070 over night at 4°C). Detection shown was using DyLight549 Donkey anti-Rabbit IgG (611-742-127 lot 21100, shown as green)   DyLight 488 Donkey anti-Mouse IgG (610-741-124 lot 21095, shown as blue),  and DyLight 649 Donkey anti-Goat IgG (605-743-125 lot 20834, shown as red) at 1:10000 (in MB-070 30 min RT). Blots were washed, rinsed in methanol, dried and Images were collected using the BioRad VersaDoc System.
WB Image
Goat anti Alpha-1anti-Trypsin antibody (200-101-147 lot 5854) was used to detect Alpha-1anti-Trypsin under reducing (R) conditions. Reduced sample of purified target protein contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT). Images were collected using the BioRad VersaDoc System.
WB Image
primary and Dylight conjugated secondary antibodies were used to detect: Human transferrin (1° 109-4134, green 2° 611-743-127); Alpha 1 anti trypsin (1° TA319139, red 2° 605-742-125); and Human IgG (1° 109-3102, Blue 2° 610-741-124 in a multiplex fluorescent western blot of human serum. Each primary antibody was diluted to 1:1000 in IRdye blocking buffer (MB-070) and incubated for 2 hrs at RT. Blot was 3X in TTBS, 1X in TBS and probed with secondary antibodies diluted 1:10000) in IRdye blocking buffer and incubated ~ 1hr at 4 degrees. After wash 2X in TTBS and 2X in TBS, blot was rinsed 2X in MeOH, dried and imaged using the Biorad VersaDoc4000.

 

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