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Home Antibody All anti-JAG1 antibodies

Anti-JAG1 Antibody

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SKU Description Amount Price Availability*  
TA319134 Rabbit polyclonal anti-Jagged 1 antibody 500ug $325 3-7 Days
LC400079 JAG1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenThis protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 110-125 of human Jagged-1protein.
Clone Name IsotypeIgG
Species ReactivityHuman, mouse Concentration1.0 mg/mL
Guaranteed Application *WB, IHC, IF Suggested DilutionsELISA: 1:20,000 - 1:100,000, WB: 1:500 - 1:2,000, IHC: 1:100 - 1:500, IF: 1:200 - 1:1,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Anti Jagged 1 Antibody recognizes the jagged 1 protein encoded by the JAG1 gene, that is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signaling through notch 1 has also been shown to play a role in hematopoies.

Reference Data

Target NameHomo sapiens jagged 1 (JAG1)
Alternative NameAGS; AHD; AWS; CD339; HJ1; JAGL1
Database LinkNP_000205
Entrez Gene 182 Human
Entrez Gene 16449 Mouse
Function
Related PathwayES Cell Differentiation/IPSTransmembraneDruggable Genome Notch signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot using Protein A purified anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5).  Lane 4 contained sample buffer only.  The band at ~134 kDa in lane 5 is believed to be Jagged-1 precursor.  The identity of minor reactive bands is unknown.  Each lane contains approximately 20 µg of lysate.  Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG.  Exposure time was 1 min.  Predicted molecular weight is 134 kDa.
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Immunohistochemical staining of human cervical cancer tissue (40X magnification) using Protein A purified anti-Jagged-1 antibody. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:100 dilution of primary antibody was used. Personal Communication. Martin Kast Laboratory.
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Immunofluorescence microscopy using Protein A purified anti-Jagged-1 antibody of human corneal epithelial cells.  Primary antibody was used at a 1:500 dilution. The Jagged1 (green staining) is local-ized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue).  Personal Communication.  Aihua Ma, Univdersity of Cardiff.
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