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Anti-MED1 PHOSPHO Antibody
Also for MED1 (NM_004774)
|The antiserum was produced against synthesized phosphopeptide derived from human PPAR-ß around the phosphorylation site of threonine 1457 (A-Y-TP-P-Q).|
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:1000
|Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|PPAR-ÃŸ (Phospho-Thr1457) antibody detects endogenous levels of PPAR-ÃŸ only when phosphorylated at threonine 1457.
|Homo sapiens mediator complex subunit 1 (MED1)|
|CRSP1; CRSP200; DRIP205; DRIP230; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2|
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Western blot - PPAR-ß/MED1 (phospho T1457) antibody. All lanes : Anti-PPAR-ß/MED1 (phospho T1457) antibody - ChIP Grade at 1/500 dilution. Lane 1 : HuvEc cell extracts treated with Serum (20%, 30mins). Lane 2 : HuvEc cell extracts treated with Serum (20%, 30mins) with immunising peptide. Predicted band size : 180 kD. Observed band size : 170 kDa.
Immunohistochemistry analysis of paraffin-embedded human heart tissue using PPAR-ß (Phospho-Thr1457) antibody. Left: without immunizing phosphopeptide; Right: with immunizing phosphopeptide.
Immunocytochemistry/ Immunofluorescence - PPAR-ß/MED1 (phospho T1457) antibody. at 1/500 - 1/1000 dilution staining PPAR-ß/MED1 in HeLa cells by Immunofluorescence. Left: without immunizing phosphopeptide; Right: with immunizing phosphopeptide.