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Also for RRN3 (NM_018427)
|The antiserum was produced against synthesized non-phosphopeptide derived from human TIF-IA around the phosphorylation site of serine 649 (Q-P-SP-P-L).|
||WB: 1:500~1:3000, ELISA: 1:1000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|TIF-IA (Ab-649) antibody detects endogenous levels of total TIF-IA protein.
|Homo sapiens RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae) (RRN3)|
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Western blot analysis of extracts from HeLa cells, treated with calyculinA (50ng/ml, 30mins), using TIF-IA (Ab-649) antibody.The lane on the right is treated with the synthesized peptide.