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Anti-TNK2 PHOSPHO Antibody
Also for TNK2 (NM_005781)
|The antiserum was produced against synthesized phosphopeptide derived from human ACK1 around the phosphorylation site of tyrosine 284 (D-H-YP-V-M).|
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:10000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|ACK1 (Phospho-Tyr284) antibody detects endogenous levels of ACK1 only when phosphorylated at tyrosine 284.
|Homo sapiens tyrosine kinase, non-receptor, 2 (TNK2), transcript variant 1|
|ACK; ACK-1; ACK1; p21cdc42Hs|
EGFR1 Signaling Pathway
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Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using ACK1 (Phospho-Tyr284) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of A549 cells, using ACK1 (Phospho-Tyr284) antibody.The picture on the right is treated with the synthesized peptide.