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Anti-ESPL1 PHOSPHO Antibody
Also for ESPL1 (NM_012291)
|The antiserum was produced against synthesized phosphopeptide derived from human SEPARASE around the phosphorylation site of serine 801 (N-S-SP-P-V).|
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:40000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|SEPARASE (Phospho-Ser801) antibody detects endogenous levels of SEPARASE only when phosphorylated at serine 801.
|Homo sapiens extra spindle pole bodies homolog 1 (S. cerevisiae) (ESPL1)|
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Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using SEPARASE (Phospho-Ser801) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SEPARASE (Phospho-Ser801) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HuvEc cells, using SEPARASE (Phospho-Ser801) antibody.The picture on the right is treated with the synthesized peptide.