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Anti-YWHAZ PHOSPHO Antibody
Also for YWHAZ (NM_145690)
|The antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 ? around the phosphorylation site of serine 58 (R-S-SP-W-R)|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:500~1000, IHC: 1:50~100, IF: 1:100~1:500, ELISA: 1:40000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|14-3-3 . (phospho-Ser58) antibody detects endogenous levels of 14-3-3 . only when phosphorylated at serine 58.
|Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (YWHAZ), transcript variant 2|
|14-3-3-zeta; KCIP-1; YWHAD|
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Western blot analysis of extracts from NIH/3T3 cells treated with UV (30mins), using 14-3-3 ? (phospho-Ser58) antibody.The lane on the right is treated with the synthesized peptide.Western blot analysis of extracts from A549 cells (Lane 2) and 3T3 cells (Lane 3), using 14-3-3 ? (Phospho-Ser58) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 ? (phospho-Ser58) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using 14-3-3 ? (phospho-Ser58) antibody.The picture on the right is treated with the synthesized peptide.