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Anti-SRF PHOSPHO Antibody
|The antiserum was produced against synthesized phosphopeptide derived from human SRF around the phosphorylation site of serine 99 (S-L-SP-E-M).|
|Human: Ser103; Mouse: Ser99; Rat: Ser99
||WB: 1:500~3000, IHC: 1:50~100, ELISA: 1:5000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|SRF (Phospho-Ser99) antibody detects endogenous levels of SRF only when phosphorylated at serine 99.
|Homo sapiens serum response factor (c-fos serum response element-binding transcription factor) (SRF), transcript variant 1|
MAPK signaling pathway
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Western blot analysis of extracts from LOVO cells treated with serum (20%, 15mins), using SRF (Phospho-Ser99) antibody (#TA314567).The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using SRF (Phospho-Ser99) antibody (#TA314567).The picture on the right is treated with the synthesized peptide.