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Also for MITF (NM_000248)
|The antiserum was produced against synthesized non-phosphopeptide derived from human MITF around the phosphorylation site of serine 180/73 (P-N-SP-P-M).|
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:10000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|MITF (Ab-180/73) antibody detects endogenous levels of total MITF protein.
|Homo sapiens microphthalmia-associated transcription factor (MITF), transcript variant 4|
|bHLHe32; CMM8; MI; WS2; WS2A|
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Western blot analysis of extracts from HepG2 cells (lane 1) and COLO205 cells (lane 2), using MITF (Ab-180/73) antibody.The lane on the right is treated with the synthesized peptide.Western blot analysis of extracts from HepG2 cells (Lane 2) and 3T3 cells (Lane 3), using MITF (Ab-180/73) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human skin tissue using MITF (Ab-180/73) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using MITF (Ab-180/73) antibody.The picture on the right is treated with the synthesized peptide.