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Anti-RUNX1 PHOSPHO Antibody
Also for RUNX1 (NM_001754)
|The antiserum was produced against synthesized phosphopeptide derived from human AML1 around the phosphorylation site of serine 435 (S-N-SP-P-T).|
|Human, Mouse, Rat
||WB: 1:500~1:3000, ELISA: 1:10000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|AML1 (Phospho-Ser435) antibody detects endogenous levels of AML1 only when phosphorylated at serine 435.
|Homo sapiens runt-related transcription factor 1 (RUNX1), transcript variant 1|
|AML1; AML1-EVI-1; AMLCR1; CBFA2; EVI-1; PEBP2aB|
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Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using AML1 (Phospho-Ser435) antibody.The lane on the right is treated with the synthesized peptide.Western blot analysis of extracts from 293 cells (Lane 2), HeLa cells (Lane 3) and HepG2 cells (Lane 4), using AML1 (Phospho-Ser435) Antibody. The lane on the left is treated with synthesized peptide.