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Anti-KRT8 PHOSPHO Antibody
Also for KRT8 (NM_002273)
|The antiserum was produced against synthesized phosphopeptide derived from human Cytokeratin 8 around the phosphorylation site of serine 431 (L-T-SP-P-G)|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:40000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|Cytokeratin 8 (Phospho-Ser431) antibody detects endogenous levels of Cytokeratin 8 only when phosphorylated at serine 431.
|Homo sapiens keratin 8 (KRT8), transcript variant 2|
|CARD2; CK-8; CK8; CYK8; K2C8; K8; KO|
EGFR1 Signaling Pathway
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Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using Cytokeratin 8 (Phospho-Ser431) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Cytokeratin 8 (Phospho-Ser431) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Cytokeratin 8 (Phospho-Ser431) antibody.The picture on the right is treated with the synthesized peptide.