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Anti-AURKB PHOSPHO Antibody
Also for AURKB (NM_004217)
|The antiserum was produced against synthesized phosphopeptide derived from human AurB around the phosphorylation site of threonine 232 (R-K-TP-M-C).|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:20000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|AurB (Phospho-Thr232) antibody detects endogenous levels of AurB only when phosphorylated at threonine 232.
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Western blot analysis of extracts from COS7 cells, treated with Nocodazole (1ug/ml, 16hours), using AurB (Phospho-Thr232) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using AurB (Phospho-Thr232) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HepG2 cells, using AurB (Phospho-Thr232) antibody.The picture on the right is treated with the synthesized peptide.