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Also for SMAD1 (NM_005900)
|The antiserum was produced against synthesized non-phosphopeptide derived from human Smad1 around the phosphorylation site of serine 465 (I-S-S-V-SP).|
|Human, Mouse, Rat
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:5000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|Smad1 (Ab-465) antibody detects endogenous levels of total Smad1 protein.
|Homo sapiens SMAD family member 1 (SMAD1), transcript variant 1|
|BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1|
Delta-Notch Signaling Pathway
TGF Beta Signaling Pathway
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Western blot analysis of extracts from COS7 cells, treated with EGF (200ng/ml, 15mins), using Smad1 (Ab-465) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Smad1 (Ab-465) antibody.The picture on the right is treated with the synthesized peptide.