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Anti-KCNIP3 PHOSPHO Antibody
Also for KCNIP3 (NM_013434)
|The antiserum was produced against synthesized phosphopeptide derived from human Calsenilin/KCNIP3 around the phosphorylation site of serine 63 (D-S-SP-D-S)|
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:20000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|Calsenilin/KCNIP3 (Phospho-Ser63) antibody detects endogenous levels of Calsenilin/KCNIP3 only when phosphorylated at serine 63
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Western blot analysis of extracts from 293 cells treated with PMA (125ng/ml, 30mins) and K562 cells treated with forskolin (40nM, 30mins) , using Calsenilin/KCNIP3 (Phospho-Ser63) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Calsenilin/KCNIP3 (Phospho-Ser63) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Calsenilin/KCNIP3 (Phospho-Ser63) antibody.The picture on the right is treated with the synthesized peptide.