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Anti-CCNB1 PHOSPHO Antibody
Also for CCNB1 (NM_031966)
|The antiserum was produced against synthesized phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 (T-A-SP-P-S).|
|Human: Ser126, Mouse: Ser123
||WB: 1:500~3000, IHC: 1:50~100, ELISA: 1:5000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|Cyclin B1 (Phospho-Ser126) antibody detects endogenous levels of Cyclin B1 only when phosphorylated at serine 126.
|Homo sapiens cyclin B1 (CCNB1)|
Hedgehog signaling pathway
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Western blot analysis of extracts from NIH/3T3 cells, treated with EGF (200ng/ml, 15mins), using Cyclin B1 (Phospho-Ser126) antibody (#A0068).The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Cyclin B1 (Phospho-Ser126) antibody (#A0068).The picture on the right is treated with the synthesized peptide.