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Anti-EGFR PHOSPHO Antibody
Also for EGFR (NM_005228)
|The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of threonine 678 (K-R-TP-L-R).|
||WB: 1:300~1:3000, IF: 1:100~1:500, ELISA: 1:40000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|EGFR (phospho-Thr678) antibody detects endogenous levels of EGFR only when phosphorylated at threonine 678.
|Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1|
|ERBB; ERBB1; HER1; mENA; PIG61|
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot analysis of extracts from cos7 cells treated with EGF (200ng/ml, 30mins), using EGFR (phospho-Thr678) antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HuvEc cells, using EGFR (phospho-Thr678) antibody.The picture on the right is treated with the synthesized peptide.