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Anti-EGFR PHOSPHO Antibody

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SKU Description Amount Price Availability*  
TA312532 Rabbit polyclonal EGFR (Tyr1172) antibody(Phospho-specific) 100ul $325 In Stock
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WB(2)

OriGene Data

ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine 1172 (P-D-YP-Q-Q).
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration1mg/ml
Guaranteed Application *WB Suggested DilutionsWB: 1:500~1:3000, ELISA: 1:10000
BufferPhosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Note EGFR (phospho-Tyr1172) antibody detects endogenous levels EGFR only when phosphorylated at tyrosine 1172.

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of extracts from A431 cells untreated or treated with EGF (40µM, 10mins), using EGFR (phospho-Tyr1172) antibody (Line1 and 2).The lane on the right is treated with the synthesized peptide.
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Western blot analysis of extracts from cos-7 cells (Lane 2), using EGFR (Phospho-Tyr1172) Antibody. The lane on the left is treated with synthesized peptide.
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