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Anti-RPS6KA5 PHOSPHO Antibody
Also for RPS6KA5 (NM_004755)
|The antiserum was produced against synthesized phosphopeptide derived from human MSK1 around the phosphorylation site of serine 212 (A-Y-SP-F-C).|
|Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%)
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:40000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|MSK1 (Phospho-Ser212) antibody detects endogenous levels of MSK1 only when phosphorylated at serine 212.
|Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 5 (RPS6KA5), transcript variant 1|
|MSK1; MSPK1; RLPK|
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot analysis of extracts from 3T3 cells, treated with EGF (200ng/ml, 5mins), using MSK1 (Phospho-Ser212) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from colo cells (Lane 2), using MSK1 (Phospho-Ser212) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MSK1 (Phospho-Ser212) antibody.The picture on the right is treated with the synthesized peptide.