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Also for CASP3 (NM_032991)
|The antiserum was produced against synthesized peptide derived from human Caspase 3.|
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:20000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|Caspase 3 (cleaved-Asp175) antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.
|Homo sapiens caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta|
|CPP32; CPP32B; SCA-1|
MAPK signaling pathway
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Western blot analysis of extracts from HeLa cells, treated with Etoposide (25uM, 60mins), using Caspase 3 (cleaved-Asp175) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 3 (cleaved-Asp175) antibody.The picture on the right is treated with the synthesized peptide.