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Home Antibody All anti-HSP90AB1 antibodies

Anti-HSP90AB1 PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA312343
  • Rabbit polyclonal HSP90B (Ser254) antibody(Phospho-specific)
100ul 325 In Stock
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WB(2)
IHC(1)
IF(1)
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Also for HSP90AB1 (NM_007355)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-SP-D-E).
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500~3000, IHC: 1:50~100, IF: 1:100~1:500, ELISA: 1:40000
BufferPhosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Note HSP90B (phospho-Ser254) antibody detects endogenous levels of HSP90B only when phosphorylated at serine 254.

Reference Data

Target NameHomo sapiens heat shock protein 90kDa alpha (cytosolic), class B member 1 (HSP90AB1), transcript variant 2
Alternative NameD6S182; HSP84; HSP90B; HSPC2; HSPCB
Database LinkNP_031381
Entrez Gene 3326 Human
Entrez Gene 15516 Mouse
Entrez Gene 301252 Rat
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of extracts from Hela cells treated with TNF-a (10ng/ml, 30mins), using HSP90B (phospho-Ser254) antibody.The lane on the right is treated with the synthesized peptide.
WB Image
Western blot analysis of extracts from JK cells (Lane 2) and K562 cells (Lane 3), using HSP90B (Phospho-Ser254) Antibody. The lane on the left is treated with synthesized peptide.
IHC Image
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90B (phospho-Ser254) antibody.The picture on the right is treated with the synthesized peptide.
IF Image
Immunofluorescence analysis of HeLa cells, treated with TNF-a (20nM, 15mins), using HSP90B (phospho-Ser254) antibody (#11313).The picture on the right is treated with the synthesized peptide.

 

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