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Anti-HSP90AB1 PHOSPHO Antibody
Also for HSP90AB1 (NM_007355)
|The antiserum was produced against synthesized phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-SP-D-E).|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:500~3000, IHC: 1:50~100, IF: 1:100~1:500, ELISA: 1:40000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|HSP90B (phospho-Ser254) antibody detects endogenous levels of HSP90B only when phosphorylated at serine 254.
|Homo sapiens heat shock protein 90kDa alpha (cytosolic), class B member 1 (HSP90AB1), transcript variant 2|
|D6S182; HSP84; HSP90B; HSPC2; HSPCB|
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Western blot analysis of extracts from Hela cells treated with TNF-a (10ng/ml, 30mins), using HSP90B (phospho-Ser254) antibody.The lane on the right is treated with the synthesized peptide.Western blot analysis of extracts from JK cells (Lane 2) and K562 cells (Lane 3), using HSP90B (Phospho-Ser254) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90B (phospho-Ser254) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, treated with TNF-a (20nM, 15mins), using HSP90B (phospho-Ser254) antibody (#11313).The picture on the right is treated with the synthesized peptide.