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Also for SMAD3 (NM_005902)
|The antiserum was produced against synthesized non-phosphopeptide derived from human Smad2/3 around the phosphorylation site of threonine 8 (P-F-TP-P-P).|
|Human, Mouse, Rat
||WB: 1:500~1:3000, ELISA: 1:10000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|Smad2/3 (Ab-8) antibody detects endogenous levels of total Smad2/3 protein.
|Homo sapiens SMAD family member 3 (SMAD3), transcript variant 1|
|HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3|
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot analysis of extracts from COS7 cells treated (lane 1) and untreated (lane 2) with UV (15mins), and Jurkat cells treated with EGF (200 ng/ml, 30mins) (lane 3), using Smad2/3 (Ab-8) antibody.The lane on the right is treated with the synthesized peptide.Western blot analysis of extracts from A549 cells (Lane 2) and HepG2 cells (Lane 3), using Smad2/3 (Ab-8) Antibody. The lane on the left is treated with synthesized peptide.