Homo sapiens SMAD family member 3 (SMAD3), transcript variant 1
Synonyms:
HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human Smad2/3 around the phosphorylation site of threonine 8 (P-F-TP-P-P).
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clone Name
Isotype
IgG
Species Reactivity
Human, Mouse, Rat
Concentration
1mg/ml
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
Guaranteed Application *
WB
Suggested Dilutions
WB: 1:500~1:3000, ELISA: 1:10000
Background
Smad2/3 (Ab-8) antibody detects endogenous levels of total Smad2/3 protein.
Related Pathway
Adherens junction
Adipogenesis
Cell cycle
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway
* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot analysis of extracts from COS7 cells treated (lane 1) and untreated (lane 2) with UV (15mins), and Jurkat cells treated with EGF (200 ng/ml, 30mins) (lane 3), using Smad2/3 (Ab-8) antibody.The lane on the right is treated with the synthesized peptide.Western blot analysis of extracts from A549 cells (Lane 2) and HepG2 cells (Lane 3), using Smad2/3 (Ab-8) Antibody. The lane on the left is treated with synthesized peptide.
Researchers who bought this antibody have also purchased:
