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Anti-VIM PHOSPHO Antibody
|The antiserum was produced against synthesized phosphopeptide derived from human Vimentin around the phosphorylation site of serine 56 (A-S-SP-P-G).|
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:20000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|Vimentin (Phospho-Ser56) antibody detects endogenous levels of Vimentin only when phosphorylated at serine 56.
|Homo sapiens vimentin (VIM)|
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Western blot analysis of extracts from A549 cells, treated with Nocodazole (1ug/ml, 16hours), using Vimentin (Phospho-Ser56) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Vimentin (Phospho-Ser56) antibody.The picture on the right is treated with the synthesized peptide.