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Anti-MAP2K6 PHOSPHO Antibody
Also for MAP2K6 (NM_002758)
|The antiserum was produced against synthesized phosphopeptide derived from human MKK6 around the phosphorylation site of Serine207(V-D-SP-V-A).|
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:5000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|MKK6 (Phospho-Ser207) Antibody detects endogenous levels of MKK6 only when phosphorylated at Serine207.
|Homo sapiens mitogen-activated protein kinase kinase 6 (MAP2K6)|
|MAPKK6; MEK6; MKK6; PRKMK6; SAPKK-3; SAPKK3|
MAPK signaling pathway
TGF Beta Signaling Pathway
Toll-like receptor signaling pathway
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Western blot analysis of the extracts from MDA-MB- 435 cells treated or untreated with UV using MKK3 (Ab-207) Antibody (#B7153) and MKK6 (Phospho-Ser207) Antibody (# A7153). Line1: Using # B7153; Line2: Using # B7153; Line3: Using # A7153; Line4: Using # A7153.The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin- embedded breast carcinoma, using MKK6 (Phospho- Ser207) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.The picture on the right is treated with the synthesized peptide.