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Anti-PRKCZ PHOSPHO Antibody
Also for PRKCZ (NM_002744)
|The antiserum was produced against synthesized phosphopeptide derived from human PKC? around the phosphorylation site of threonine 410 (T-S-TP-F-C).|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:500~3000, IHC: 1:50~100, IF: 1:100~1:500, ELISA: 1:20000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|PKC. (phospho-Thr410) antibody detects endogenous levels of PKC. only when phosphorylated at threonine 410.
|Homo sapiens protein kinase C, zeta (PRKCZ), transcript variant 1|
EGFR1 Signaling Pathway
MAPK signaling pathway
Wnt Signaling Pathway
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Western blot analysis of extracts from NIH/3T3 cells treated with PMA (125ng/ml, 30mins), using PKC? (phospho-Thr410) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKC? (phospho-Thr410) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using PKC? (phospho-Thr410) antibody.The picture on the right is treated with the synthesized peptide.