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Anti-MUC1 PHOSPHO Antibody
Also for MUC1 (NM_002456)
|The antiserum was produced against synthesized phosphopeptide derived from human CD227/MUC1 around the phosphorylation site of tyrosine 1229 (S-P-YP-E-K)|
|Human (Identities = 100%, Positives = 100%)
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:1000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|CD227/MUC1 (Phospho-Tyr1229) antibody detects endogenous levels of CD227/MUC1 only when phosphorylated at tyrosine 1229.
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Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using CD227/MUC1 (Phospho-Tyr1229) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using CD227/MUC1 (Phospho-Tyr1229) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HepG2 cells, using CD227/MUC1 (Phospho-Tyr1229) antibody.The picture on the right is treated with the synthesized peptide.