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Anti-IRF3 PHOSPHO Antibody
Also for IRF3 (NM_001571)
|The antiserum was produced against synthesized phosphopeptide derived from human IRF-3 around the phosphorylation site of serine 385 (G-A-SP-S-L).|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:10000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|IRF-3 (Phospho-Ser385) antibody detects endogenous levels of IRF-3 only when phosphorylated at serine 385.
|Homo sapiens interferon regulatory factor 3 (IRF3), transcript variant 1|
|MGC94729; interferon regulatory factor 3|
Toll-like receptor signaling pathway
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Western blot analysis of extracts from HT-29 cells, treated with insulin (0.01U/ml, 15mins), using IRF-3 (Phospho-Ser385) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using IRF-3 (Phospho-Ser385) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) antibody.The picture on the right is treated with the synthesized peptide.