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Anti-CHEK1 PHOSPHO Antibody
Also for CHEK1 (NM_001274)
|The antiserum was produced against synthesized phosphopeptide derived from human Chk1 around the phosphorylation site of serine 296 (I-Q-SP-N-L).|
|Human (Identities = 100%, Positives = 100%)
||WB: 1:500~1:3000, IHC: 1:50~1:100, ELISA: 1:5000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|Chk1 (Phospho-Ser296) antibody detects endogenous levels of Chk1 only when phosphorylated at serine 296.
|Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 3|
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Western blot analysis of extracts from HUVEC cells, treated with UV (15mins), using Chk1 (Phospho-Ser296) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue using Chk1 (Phospho-Ser296) antibody.The picture on the right is treated with the synthesized peptide.