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Also for CHEK1 (NM_001274)
|The antiserum was produced against synthesized non-phosphopeptide derived from human Chk1 around the phosphorylation site of serine 286 (S-E-SP-P-S)|
|Human, Mouse, Rat
||WB: 1:500~1:3000, IF: 1:100~1:500, ELISA: 1:1000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
|Chk1 (Ab-286) antibody detects endogenous levels of total Chk1 protein.
|Homo sapiens checkpoint kinase 1 (CHEK1), transcript variant 3|
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Western blot analysis of extracts from Raw264.7 cells, using Chk1 (Ab-286) antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Chk1 (Ab-286) antibody.The picture on the right is treated with the synthesized peptide.