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Also for SIRT1 (NM_012238)
|Peptide with sequence C-EKPQEVQTSRNVES, from the internal region of the protein sequence according to NP_036370.2; NP_001135970.1.|
||WB: 0.05-0.2ug/ml, IHC: 3-5ug/ml
|Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20C. Minimize freezing and thawing.|
|Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
|Homo sapiens sirtuin 1 (SIRT1), transcript variant 1|
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(0.05ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.