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Anti-ZFYVE9 Antibody EPR5020
Also for ZFYVE9 (NM_004799)
|A synthetic peptide corresponding to residues at the N-terminus of human SARA was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:10 - 1:100
|Does not react with Mouse. Is unsuitable for ICC.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for ICC.
|Homo sapiens zinc finger, FYVE domain containing 9 (ZFYVE9), transcript variant 3|
|MADHIP; NSP; SARA; SMADIP|
|SARA is a double zinc finger (FYVE domain) protein that interacts directly with SMAD2 and SMAD3, and is involved in Alzheimer's disease. SMAD proteins transmit signals from transmembrane serine/threonine kinase receptors to the nucleus. The FYVE domain has been identified in a number of unrelated signaling molecules. SARA functions to recruit SMAD2 to the transforming growth factor-beta receptor. The FYVE domain is required to maintain the normal localization of this protein but is not involved in mediating interaction with SMADs. The C-terminal domain of SARA interacts with the TGFB receptor. This protein is a component of the TGFB pathway that brings the SMAD substrate to the receptor (1). |
TGF Beta Signaling Pathway
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Western blot - Anti-SARA antibody [EPR5020]; All lanes : Anti-SARA antibody [EPR5020] at 1/10000 dilution.Lane 1 : Human heart lysate.Lane 2 : A673 cell lysate.Lane 3 : HT-1080 cell lysate.Lane 4 : BxPC-3 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 156 kDa.
Western blot - Anti-SARA antibody [EPR5020]; All lanes : Anti-SARA antibody [EPR5020] at 1/10000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : Human fetal brain lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 156 kDa.
Other-Anti-SARA antibody [EPR5020](ab124875); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARA antibody [EPR5020]; Staining of SARA in Paraffin-embedded Human kidney tissue by Immunohistochemistry using ab124875 at a dilution of 1/100.