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Anti-WAPAL Antibody EPR3407(2)
Also for WAPAL (NM_015045)
|A synthetic peptide corresponding to residues in human WAPL was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:50000 - 1:200000; IP: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IHC-P.
|Homo sapiens wings apart-like homolog (Drosophila) (WAPAL)|
|FOE; KIAA0261; WAPL|
|WAPL, also known as Wings apart-like protein homolog, is a cohesion-binding protein that regulates both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis (1, 2). Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair (1). |
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Western blot - WAPL antibody [EPR3407(2)]; All lanes : Anti-WAPL antibody [EPR3407(2)] at 1/50000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lane 3 : Jurkat cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 133 kDa.
Western blot - Anti-WAPL antibody [EPR3407(2)]; All lanes : Anti-WAPL antibody [EPR3407(2)] at 1/10000 dilution.Lane 1 : Human U2OS cells treated with siRNA control.Lane 2 : Human U2OS cells treated with siRNA #1 against WAPL. .Lane 3 : Human U2OS cells treated with siRNA #2 against WAPL.Lysates/proteins at 90 µg per lane.Secondary. Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 133 kDa.Observed band size : 150 kDa .Additional bands at : 120 kDa (possible cross reactivity),130 kDa (possible cross reactivity),145 kDa (possible cross reactivity).
Immunocytochemistry/ Immunofluorescence - WAPL antibody [EPR3407(2)]; ab109537 at 1/100 dilution staining WAPL in HeLa cells by Immunofluorescence.