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Anti-VCL Antibody EPR8185
|A synthetic peptide corresponding to residues in human Vinculin was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IHC-P.
|Homo sapiens vinculin (VCL), transcript variant 2|
|CMD1W; CMH15; MV; MVCL|
|Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in Vinculin are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia (1). |
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Western blot - Anti-Vinculin antibody [EPR8185]; All lanes : Anti-Vinculin antibody [EPR8185] at 1/10000 dilution.Lane 1 : PC3 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : U937 cell lysate.Lane 4 : K562 cell lysate.Lane 5 : HUVEC cell lysate.Lane 6 : Human fetal liver lysate.Lane 7 : Human fetal kidney lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 124 kDa.Observed band size : 124 kDa.
Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185]; ab129002, at 1/100 dilution staining Vinculin in HUVEC cells by Immunofluorescence.