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Anti-SLC4A1 Antibody EPR1426
Also for SLC4A1 (NM_000342)
|A synthetic peptide corresponding to residues in human AE1 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: Use at an assay dependent concentration
|Does not react with Mouse, Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens solute carrier family 4 (anion exchanger), member 1 (SLC4A1)|
|AE1; BND3; CD233; DI; EMPB3; EPB3; FR; RTA1A; SW; WD; WD1; WR|
|The AE1 (anion exchanger, band 3) protein is expressed in erythrocytes and in the A-type intercalated cells of the kidney distal collecting tubule. In both cell types it mediates the electroneutral transport of chloride and bicarbonate ions across the lipid bilayer, and, in erythrocytes (1). AE1 is phosphorylated on Tyr8and Tyr21 by SYK. It has been suggested that, upon phosphorylation by p72syk, Tyr8, and Tyr21 act as docking sites for the SH2 domain of Lyn, which subsequently phosphorylates AE1 at additional secondary sites (2).|
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Western blot - Band 3 antibody [EPR1426]; Anti-Band 3 antibody [EPR1426] at 1/1000 dilution + Red blood cell lysate at 10 µg.Predicted band size : 102 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Band 3 antibody [EPR1426]; ab108414, at 1/100 dilution, staining Band 3 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Band 3 antibody [EPR1426]; ab108414, at 1/100 dilution, staining Band 3 in paraffin-embedded Human lung tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-Band 3 antibody [EPR1426]; ab108414 staining Band 3 in Rhesus monkey erythroid cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in methanol and then incubated with ab108414 at a 1/100 dilution for 1 hour. The secondary used was an FITC conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.