OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-RPSA antibodies

Anti-RPSA Antibody EPR8469

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA311054
  • Rabbit Monoclonal antibody against Laminin Receptor (RPSA)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC423324) , 20ug
100ul 325 3-7 Days
Add to Shopping Cart
WB(1)
IHC(2)
FC(1)
spacer
Also for RPSA (NM_001012321)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Laminin Receptor was used as an immunogen.
Clone NameEPR8469 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250; IP: 1:10 - 1:100; FC: 1:100 - 1:1000
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens ribosomal protein SA (RPSA), transcript variant 2
Alternative Name37LRP; 67LR; ICAS; LAMBR; lamR; LAMR1; LBP; LBP/p40; LRP; LRP/LR; NEM/1CHD4; p40; SA
Database LinkNP_001012321
FunctionLaminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. Laminin Receptor is a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kDa laminin receptor, 37 kDa laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of Laminin Receptor is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor is higher in colon carcinoma tissue and a lung cancer cell line than their normal counterparts. In addition, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotypes (1).
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-67kDa Laminin Receptor antibody [EPR8469]; All lanes : Anti-67kDa Laminin Receptor antibody [EPR8469] at 1/1000 dilution.Lane 1 : K562 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : HepG2 cell lysate.Lane 4 : C6 cell lysate.Lane 5 : RAW 264.7 cell lysate.Lane 6 : PC-12 cell lysate.Lane 7 : NIH/3T3 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 33 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8469]; Immunohistochemical analysis of paraffin-embedded Human breast tissue labelling 67kDa Laminin Receptor with TA311054 at 1/100 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-67kDa Laminin Receptor antibody [EPR8469]; Immunohistochemical analysis of paraffin-embedded Human kidney tissue labellig 67kDa Laminin Receptor with TA311054 at 1/100 dilution.
FC Image
Flow Cytometry - Anti-67kDa Laminin Receptor antibody [EPR8469]; Overlay histogram showing MCF7 cells stained with TA311054 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title