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Anti-PRKCE Antibody EPR1482(2)
Also for PRKCE (NM_005400)
|A synthetic peptide corresponding to residues in human PKC Epsilon was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1:10000 - 1:50000; IHC-P: 1:100 - 1:250; FC: 1:100 - 1:500; ICC/IF: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens protein kinase C, epsilon (PRKCE)|
|Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC Epsilon is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior (1).|
Wnt Signaling Pathway
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Western blot - Anti-PKC epsilon antibody [EPR1482(2)]; All lanes : Anti-PKC epsilon antibody [EPR1482(2)] at 1/10000 dilution.Lane 1 : SH-SY5Y cell lysate.Lane 2 : MCF7 cell lysate.Lane 3 : HT29 cell lysate.Lane 4 : Jurkat cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 84 kDa.Observed band size : 82 kDa .
Other-Anti-PKC epsilon antibody [EPR1482(2)](ab124806); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC epsilon antibody [EPR1482(2)]; ab124806, at 1/100 dilution, staining PKC epsilon in paraffin-embedded Human kidney tissue by Immunohistochemistry.