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Anti-PIK3C3 Antibody EPR5301
Also for PIK3C3 (NM_002647)
|A synthetic peptide corresponding to residues in human PI3-Kinase Class 3 (VPS34) was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,ICC or IP.
|Homo sapiens phosphatidylinositol 3-kinase, catalytic subunit type 3 (PIK3C3)|
Entrez Gene 5289 Human
Entrez Gene 225326 Mouse
Entrez Gene 65052 Rat
|PI3-Kinase Class 3 (VPS34) is the catalytic subunit of the PI3K complex. It is involved in the transport of lysosomal enzyme precursors to lysosomes and the induction of autophagy during nutrient deprivation (1, 2). Thus, PI3-Kinase Class 3 (VPS34) regulates intracellular vesicular transport at multiple steps through the production of phosphatidylinositol-3-phosphate (PI(3)P). Certain downstream effectors are involved in each of the processes that PI3-Kinase Class 3 (VPS34) regulates. It exerts its function through the recruitment of proteins that contain PI(3)P-binding motifs such as FYVE or PX domains (3). PI3-Kinase Class 3 (VPS34) is also required for the activation of the mTOR (mammalian target of rapamycin)/S6K1 (S6 kinase 1) pathway, which regulates protein synthesis in response to nutrient availability (2).|
|Druggable Genome Inositol phosphate metabolismMetabolic pathwaysPhosphatidylinositol signaling systemRegulation of autophagy|
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Western blot - Anti-PI 3 Kinase Class 3 antibody [EPR5301]; All lanes : Anti-PI 3 Kinase Class 3 antibody [EPR5301] at 1/1000 dilution.Lane 1 : L6 cell lysate.Lane 2 : C2C12 cell lysate.Lane 3 : 293T cell lysate.Lane 4 : Mouse brain tissue lysate.Lane 5 : SHSY-5Y cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 102 kDa.Observed band size : 100 kDa .
Other-Anti-PI 3 Kinase Class 3 antibody [EPR5301](TA311028); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase Class 3 antibody [EPR5301]; TA311028, at 1/50 dilution, staining PI 3 Kinase in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.