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Anti-NONO Antibody EPR5269

WB(1)

IHC(1)

Gene Name Homo sapiens non-POU domain containing, octamer-binding (NONO), transcript variant 1 Synonyms: NMT55; NRB54; P54; P54NRB Immunogen A synthetic peptide corresponding to residues in human p54nrb was used as an immunogen. Buffer Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt. Clone Name EPR5269 Isotype Species Reactivity Human, Mouse Concentration Purification (Protein A or G Sepharose) Guaranteed Application * WB, IHC Suggested Dilutions WB: 1:1,000 - 10,000; Background p54nrb (NONO) is a DNA- and RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing (1). As part of the splicesomal complex, PTB-associated RNA splicing factor (PSF) and p54nrb are essential for both step I and II pre-mRNA splicing reactions (2). p54nrb is a component of the SFPQ-NONO complex, which is associated with MATR3 and plays a role in nuclear retention of defective RNA’s. The complex is involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 (3). A rearrangement between p54nrb and the transcription factor E3 has been observed in papillary renal cell carcinoma (1). Related Pathway

Related Lysate from OriGene • Transient overexpression lysate of non-POU domain containing, octamer-binding (NONO), transcript variant 1 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • NONO - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • NONO - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Nono - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • NONO - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Nono - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens non-POU domain containing, octamer-binding (NONO), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human non-POU domain containing, octamer-binding (NONO), transcript variant 1, mGFP tagged • GFP-tagged ORF clone of Homo sapiens non-POU domain containing, octamer-binding (NONO), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human non-POU domain containing, octamer-binding (NONO), transcript variant 1 , Myc-DDK-tagged Related cDNA Clones • Homo sapiens non-POU domain containing, octamer-binding (NONO), transcript variant 1, mRNA

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

• HRP
• Biotin
• PE
• FITC
• DyLight® 488
• DyLight® 550
• DyLight® 594
• DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)