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Home Antibody All anti-NKX2-1 antibodies

Anti-NKX2-1 Antibody EPR8190

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA311007
  • Rabbit Monoclonal antibody against TTF1 (NKX2-1)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC421530) , 20ug
100ul 325 3-7 Days
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WB(2)
IHC(8)
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Also for NKX2-1 (NM_001079668)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human TTF1 was used as an immunogen.
Clone NameEPR8190 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:500 - 1:1000; IP: 1:10 - 1:100
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or ICC.

Reference Data

Target NameHomo sapiens NK2 homeobox 1 (NKX2-1), transcript variant 1
Alternative NameBCH; BHC; NK-2; NKX2.1; NKX2A; TEBP; TITF1; TTF-1; TTF1
Database LinkNP_001073136
FunctionThe thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that activates the transcriptional activity of thyroid-specific gene promoters by binding to them. Hence, TTF-1 is crucial in the maintenance of the thyroid differentiation phenotype (1). Thyroid dysgenesis (TD) is responsible for most cases of congenital hypothyroidism, a condition that affects about one in 4000 newborns. Mutations in PAX8, TTF1, or FOXE1 may account for congenital hypothyroidism in patients with either isolated TD or TD with associated malformations involving kidney, lung, forebrain, and palate (2). In addition to its presence in thyroid gland epithelium, the human TTF-1 protein was detected by immunohistochemistry in human fetal lung as early as 11 weeks of gestation, being localized in the nuclei of epithelial cells of the developing airways. After birth, TTF-1 was selectively expressed in Type II epithelial cells in the alveoli and in subsets of bronchiolar epithelial cells in the conducting regions of the lung (3).
Related Pathway

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WB Image
Western blot - Anti-TTF1 antibody [EPR8190]; All lanes : Anti-TTF1 antibody [EPR8190] at 1/1000 dilution.Lane 1 : HeLa lysate.Lane 2 : Human lung adenocarcinoma lysate.Lane 3 : Fetal lung lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 39 kDa.
WB Image
Western blot - Anti-TTF1 antibody [EPR8190]; All lanes : Anti-TTF1 antibody [EPR8190] at 1/1000 dilution.Lane 1 : Mouse lung lysate.Lane 2 : Rat lung lysate .Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 39 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190]; Immunohistochemical analysis of paraffin-embedded Human papillary thyroid carcinoma tissue labelling TTF1 with TA311007 at 1/500 dilution
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTF1 antibody [EPR8190]; Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling TTF1 with TA311007 at 1/500 dilution
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-TTF1 antibody [EPR8190](TA311007); TA311007 showing positive staining in Lung adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-TTF1 antibody [EPR8190](TA311007); TA311007 showing positive staining in Thyroid gland tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-TTF1 antibody [EPR8190](TA311007); TA311007 showing negative staining in Normal liver tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-TTF1 antibody [EPR8190](TA311007); TA311007 showing positive staining in Small cell lung carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-TTF1 antibody [EPR8190](TA311007); TA311007 showing negative staining in Lung squamous carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-TTF1 antibody [EPR8190](TA311007); TA311007 showing negative staining in Normal lung tissue.

 

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