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Home Antibody All anti-NCL antibodies

Anti-NCL Antibody EPR7952

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310997
  • Rabbit Monoclonal antibody against Nucleolin (NCL)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417337) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
Assay(1)
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Also for NCL (NM_005381)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Nucleolin was used as an immunogen.
Clone NameEPR7952 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsWB: 1:10000 - 1:50000; IHC-P: 1:250 - 1:500; ICC/IF: 1:100 - 1:250
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens nucleolin (NCL)
Alternative NameC23
Database LinkNP_005372
FunctionNucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Nucleolin induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. Nucleolin also plays a role in the process of transcriptional elongation (1).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Nucleolin antibody [5698-1]; All lanes : Anti-Nucleolin antibody [EPR7952] at 1/10000 dilution.Lane 1 : Fetal brain lysate.Lane 2 : K562 cell lysate.Lane 3 : HuT-78 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 76 kDa.Observed band size : 100 kDa .
Assay Image
Other-Anti-Nucleolin antibody [EPR7952](TA310997); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952]; TA310997, at 1/250 dilution, staining Nucleolin in paraffin-embedded Human colon tissue by Immunohistochemistry.

 

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