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Home Antibody All anti-NCL antibodies

Anti-NCL Antibody EPR7952

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310997
  • Rabbit Monoclonal antibody against Nucleolin (NCL)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417337) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
Assay(1)
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Also for NCL (NM_005381)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Nucleolin was used as an immunogen.
Clone NameEPR7952 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsWB: 1:10000 - 1:50000; IHC-P: 1:250 - 1:500; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 76 kDa
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens nucleolin (NCL)
Alternative NameC23
Database LinkNP_005372
Entrez Gene 4691 Human
Entrez Gene 17975 Mouse
Entrez Gene 25135 Rat
FunctionNucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Nucleolin induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. Nucleolin also plays a role in the process of transcriptional elongation (1).
Related PathwayStem cell - PluripotencyDruggable Genome Pathogenic Escherichia coli infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Nucleolin antibody [5698-1]; All lanes : Anti-Nucleolin antibody [EPR7952] at 1/10000 dilution.Lane 1 : Fetal brain lysate.Lane 2 : K562 cell lysate.Lane 3 : HuT-78 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 76 kDa.Observed band size : 100 kDa .
Assay Image
Other-Anti-Nucleolin antibody [EPR7952](TA310997); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nucleolin antibody [EPR7952]; TA310997, at 1/250 dilution, staining Nucleolin in paraffin-embedded Human colon tissue by Immunohistochemistry.

 

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