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Anti-LGALS8 Antibody EPR4857
Also for LGALS8 (NM_006499)
|A synthetic peptide corresponding to residues in human Galectin-8 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100; FC: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens lectin, galactoside-binding, soluble, 8 (LGALS8), transcript variant 1|
|Gal-8; PCTA-1; PCTA1; Po66-CBP|
|Galectin-8 is a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. Galectin-8 is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions (1).|
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Western blot - Galectin 8 antibody [EPR4857]; All lanes : Anti-Galectin 8 antibody [EPR4857] at 1/1000 dilution.Lane 1 : HepG2 cell lysate.Lane 2 : LNCaP cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 40 kDa.
Western blot ; Anti-Galectin 8 antibody [EPR4857] at 1/5000 dilution + Human Galectin 8 full length protein at 0.1 µg.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 90 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Galectin 8 antibody [EPR4857]; ab109519, at a 1/100 dilution, staining Human prostatic adenocarcinoma, using Immunohistochemstry, Formalin/PFA-fixed paraffin-embedded tissue.
Flow Cytometry-Anti-Galectin 8 antibody [EPR4857](ab109519); Overlay histogram showing HeLa cells stained with ab109519 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.