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Anti-LAMC1 Antibody EPR8217
Also for LAMC1 (NM_002293)
|A synthetic peptide corresponding to residues in human Laminin Gamma 1 was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; FC: 1:10 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for ICC,IHC-P or IP.|
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens laminin, gamma 1 (formerly LAMB2) (LAMC1)|
|Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. Laminin Gamma 1 is the gamma chain isoform laminin, gamma 1. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this protein also suggested that it had diverged considerably from the beta chain proteins. Embryos of transgenic mice in which both alleles of the gamma 1 chain gene were inactivated by homologous recombination, lacked basement membranes, indicating that Laminin Gamma 1 is necessary for laminin heterotrimer assembly (1).|
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Western blot - Anti-Laminin gamma 1 antibody [EPR8217]; All lanes : Anti-Laminin gamma 1 antibody [EPR8217] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : JAR cell lysate.Lane 3 : A431 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 178 kDa.
Flow Cytometry - Anti-Laminin gamma 1 antibody [EPR8217]; Flow Cytometry analysis of permeabilized A431 cells labelling Laminin gamma 1 with TA310967 at 1/10 dilution (red). Rabbit IgG negative control shown in green.