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Anti-ITGA2 Antibody EPR5788
Also for ITGA2 (NM_002203)
|A synthetic peptide corresponding to residues in the extracellular domain of human CD49b/Integrin alpha-2 was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC, FC
||FC: 1:10 - 1:1000; WB: 1:10000 - 1:50000; IHC-P: 1:250 - 1:500
|Does not react with Rat. Is unsuitable for ICC/IF or IP.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Is unsuitable for ICC/IF or IP.
|Homo sapiens integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) (ITGA2), transcript variant 1|
|BR; CD49B; GPIa; HPA-5; VLA-2; VLAA2|
|CD49b/Integrin alpha-2 belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane glycoproteins composed of a distinct alpha chain and a common beta chain. They are found on a wide variety of cell types including, T cells, fibroblasts and platelets. Integrins are involved in cell adhesion and also participate in cell-surface mediated signaling (1).|
Hematopoietic cell lineage
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Western blot - Anti-Integrin alpha 2 antibody [EPR5788]; All lanes : Anti-Integrin alpha 2 [EPR5788] antibody at 1/10000 dilution.Lane 1 : T47D lysate.Lane 2 : 293T lysate.Lane 3 : Human platelet lysate.Lane 4 : A431 lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 129 kDa.Observed band size : 150 kDa .
Other-Anti-Integrin alpha 2 antibody [EPR5788](TA310955); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR5788]; Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Integrin alpha 2 with TA310955 at a dilution of 1/250.
Flow Cytometry - Anti-Integrin alpha 2 [EPR5788] antibody; Overlay histogram showing A431 cells stained with TA310955 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1Âµg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.