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Anti-USP22 Antibody EPR4352(2)
Also for USP22 (NM_015276)
|A synthetic peptide corresponding to residues in human USP22 was used as an immunogen.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IF, FC
||ICC/IF: 1:200; WB: 1:1000 - 1:5000; IP: 1:10 - 1:100; FC: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for ICC or IHC-P.
|Homo sapiens ubiquitin specific peptidase 22 (USP22)|
Entrez Gene 23326 Human
|USP22 belongs to a large family of proteins with ubiquitin hydrolase activity (1). USP22 catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator (2). It is a death-from-cancer signature and a subunit of the hSAGA coactivator complex (3). It is recruited to specific promoters by activators such as MYC, where it is required for transcription. Moreover, USP22 is required for nuclear receptor-mediated transactivation and cell cycle progression (2).|
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Western blot - USP22 antibody [EPR4352(2)]; All lanes : Anti-USP22 antibody [EPR4352(2)] at 1/1000 dilution.Lane 1 : HeLa lysate.Lane 2 : Human placenta lysate.Lane 3 : HT-1376 lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 60 kDa.
Other-Anti-USP22 antibody [EPR4352(2)](TA310932); Equilibrium disassociation constant (KD)..
Immunocytochemistry/ Immunofluorescence - Anti-USP22 antibody [EPR4352(2)]; TA310932 (1/200) staining USP22 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red).
Flow Cytometry - USP22 antibody [EPR4352(2)]; Flow cytometric analysis of permeabilized HeLa cells using 1/10 TA310932 (red) or a rabbit IgG (negative) (green).