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Anti-TSPO Antibody EPR5384
Also for TSPO (NM_000714)
|A synthetic peptide corresponding to residues in C-terminus of human TSPO was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100; FC: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Rat
|Homo sapiens translocator protein (18kDa) (TSPO), transcript variant PBR|
|BPBS; BZRP; DBI; IBP; MBR; mDRC; PBR; PBS; pk18; PKBS; PTBR|
|TSPO (Mitochondrial benzodiazepine receptor) is present mainly in the mitochondrial compartment of peripheral tissues, and it interacts with some benzodiazepines and has different affinities than its endogenous counterpart. This protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis (1).|
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Western blot - PBR antibody [EPR5384]; All lanes : Anti-PBR antibody [EPR5384] at 1/10000 dilution.Lane 1 : U-87 MG cell lysate.Lane 2 : 293T cell lysate.Lane 3 : A431 cell lysate.Lane 4 : RAW264.7 cell lysate.Lane 5 : NIH3T3 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 19 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBR antibody [EPR5384]; ab109497 at 1/100 dilution staining PBR in paraffin embedded Human colon tissue.
Immunocytochemistry/ Immunofluorescence - PBR antibody [EPR5384]; ab109497 at 1/50 dilution staining PBR in A431 cells
Flow Cytometry - Anti-PBR antibody [EPR5384]; Overlay histogram showing HepG2 cells stained with ab109497 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.