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Home Antibody All anti-TSPO antibodies

Anti-TSPO Antibody EPR5384

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA310909 Rabbit Monoclonal antibody against TSPO 100ul $325 In Stock
LC424543 TSPO HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(1)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in C-terminus of human TSPO was used as an immunogen.
Clone NameEPR5384 IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100; FC: 1:50
Predicted MW Explanation 19 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Rat

Reference Data

Target NameHomo sapiens translocator protein (18kDa) (TSPO), transcript variant PBR
Alternative NameBPBS; BZRP; DBI; IBP; MBR; mDRC; PBR; PBS; pk18; PKBS; PTBR
Database LinkNP_000705
Entrez Gene 706 Human
Entrez Gene 12257 Mouse
FunctionTSPO (Mitochondrial benzodiazepine receptor) is present mainly in the mitochondrial compartment of peripheral tissues, and it interacts with some benzodiazepines and has different affinities than its endogenous counterpart. This protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis (1).
Related PathwayTransmembraneDruggable Genome Neuroactive ligand-receptor interaction

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - PBR antibody [EPR5384]; All lanes : Anti-PBR antibody [EPR5384] at 1/10000 dilution.Lane 1 : U-87 MG cell lysate.Lane 2 : 293T cell lysate.Lane 3 : A431 cell lysate.Lane 4 : RAW264.7 cell lysate.Lane 5 : NIH3T3 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 19 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBR antibody [EPR5384]; TA310909 at 1/100 dilution staining PBR in paraffin embedded Human colon tissue.
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Immunocytochemistry/ Immunofluorescence - PBR antibody [EPR5384]; TA310909 at 1/50 dilution staining PBR in A431 cells
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Flow Cytometry - Anti-PBR antibody [EPR5384]; Overlay histogram showing HepG2 cells stained with TA310909 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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