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Anti-TRMT1 Antibody EPR8993
Also for TRMT1 (NM_017722)
|A synthetic peptide corresponding to residues in human TRMT1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:10000 - 1:50000; IHC-P: 1:50 - 1:100; ICC/IF: 1:50 - 1:100
|Preservative: 0.01% Sodium azide|
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens tRNA methyltransferase 1 homolog (S. cerevisiae) (TRMT1), transcript variant 1|
|TRMT1 dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups (1). Mutations in TRMT1 completely abrogate tRNA(m2,2G)dimethyltransferase enzyme activity (2).|
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Western blot - Anti-TRM1 antibody; All lanes : Anti-TRM1 antibody [EPR8993] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : PC3 cell lysate.Lane 3 : Jurkat cell lysate.Lane 4 : Caco 2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 72 kDa.Observed band size : 72 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRM1 antibody; Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling TRM1 with ab134965 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRM1 antibody [EPR8993]; Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labelling TRM1 with ab134965 at 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TRM1 antibody [EPR8993]; Immunofluorescent analysis of HeLa cells labelling TRM1 with ab134965 at 1/50 dilution.