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Anti-S100A4 Antibody EPR2761(2)
Also for S100A4 (NM_002961)
|A synthetic peptide corresponding to residues on the C-terminus in human S100A4 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||ICC/IF: 1:100 - 1:250; WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; FC: 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens S100 calcium binding protein A4 (S100A4), transcript variant 1|
|18A2; 42A; CAPL; FSP1; MTS1; P9KA; PEL98|
|S100A4 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and they are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 proteins include at least 13 members. S100A4 may function in motility, invasion, and tubulin polymerization. Altered expression of S100A4 have been implicated in tumor metastasis.|
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Western blot - Anti-S100A4 antibody [EPR2761(2)]; All lanes : Anti-S100A4 antibody [EPR2761(2)] at 1/1000 dilution.Lane 1 : Human tonsil lysate.Lane 2 : A549 cell lysate.Lane 3 : A375 cell lysate.Lane 4 : Human small intestine lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 12 kDa.Observed band size : 12 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)]; ab124805, at 1/250 dilution, staining S100A4 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR2761(2)]; Immunohistochemical analysis of Human lung tissue, staining S100A4 with ab124805.Tissue was fixed with HOPE and blocked with blocking solution for 5 minutes at 25°C. Samples were incubated with primary antibody (1/1000 in diluent) for 1 hour at 25°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR2761(2)]; ab124805 staining S100A4 in the A549 cell line from Human lungs by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with Triton X-100 0.25% in PBS. Samples were incubated with primary antibody (1/100) for45 minutesat 25°C. A TRITC-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
Flow Cytometry - Anti-S100A4 antibody [EPR2761(2)]; Overlay histogram showing HeLa cells stained with ab124805 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.