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Anti-RNF14 Antibody EPR9041
Also for RNF14 (NM_183401)
|A synthetic peptide corresponding to residues in human RNF14 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, FC
||WB: 1:1000 - 1:10000; FC: 1:10 - 1:100
|Preservative: 0.01% Sodium azide|
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC/IF,IHC-P or IP.
|Homo sapiens ring finger protein 14 (RNF14), transcript variant 5|
|ARA54; HFB30; HRIHFB2038; TRIAD2|
|RNF14 contains a RING zinc finger, a motif known to be involved in protein-protein interactions. It interacts with androgen receptor (AR) and may function as a coactivator that induces AR target expression in prostate. A dominant negative mutant of RNF14 has been demonstrated to inhibit the AR-mediated growth of prostate cancer. It also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins (1).|
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Western blot - Anti-RNF14 antibody [EPR9041]; All lanes : Anti-RNF14 antibody [EPR9041] at 1/1000 dilution.Lane 1 : LNCaP cell lysate.Lane 2 : K562 cell lysate.Lane 3 : Jurkat cell lysate.Lane 4 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat-anti-rabbit HRP at 1/2000 dilution.Predicted band size : 54 kDa.
Other-Anti-RNF14 antibody [EPR9041](ab134927); Equilibrium disassociation constant (KD)..
Flow Cytometry - Anti-RNF14 antibody [EPR9041]; Flow cytometric analysis of permeabilized K562 cells labelling RNF14 with ab134927 at 1/10 dilution (red) or a non-specific rabbit IgG (green).