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Home Antibody All anti-RANGAP1 antibodies

Anti-RANGAP1 Antibody EPR3295

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA310812 Rabbit Monoclonal antibody against RanGAP1 100ul $325 In Stock
LC401016 RANGAP1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for RANGAP1 (NM_002883)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human RanGAP1 was used as an immunogen.
Clone NameEPR3295 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1,000 - 5,000; IHC 1:100 - 250; ICC: 1:100 - 250;
BufferStore at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Reference Data

Target NameHomo sapiens Ran GTPase activating protein 1 (RANGAP1), transcript variant 2
Alternative NameFug1; RANGAP; SD
Database LinkNP_002874
Entrez Gene 5905 Human
Entrez Gene 19387 Mouse
Entrez Gene 362965 Rat
FunctionRanGAP1 is the activator of the Ras-related nuclear GTPase Ran, which is involved in the nucleocytoplasmic transport, mitotic spindle assembly, cell cycle control and nuclear envelope (NE) formation (1, 2). Its roles are accomplished by the asymmetric distribution of its GTP- and GDP-bound forms, enabled by the specific localization of Ran accessory proteins, the Ran GTPase-activating protein RanGAP and the nucleotide exchange factor RCC. Mammalian RanGAP1 is targeted to the NE during interphase and to the spindle and kinetochores during mitosis via SUMOylated C-terminal domain and interaction with the nucleoporin Nup358/RanBP2 (2). RanGAP1 is highly expressed in brain, thymus and testis (3).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - RanGAP1 antibody [EPR3295]; All lanes : Anti-RanGAP1 antibody [EPR3295] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : MCF-7 cell lysate.Lane 3 : SH-SY5Y cell lysate.Lane 4 : A549 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit antibody at 1/2000 dilution.Predicted band size : 64 kDa.Observed band size : 64 kDa.Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RanGAP1 antibody [EPR3295]; b92360 at 1/100 dilution staining RanGAP1 in paraffin-embedded (1) Human breast carcinoma tissue and (2) Human testis tissue by immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - RanGAP1 antibody [EPR3295]; TA310812 at 1/100 dilution staining RanGAP1 in HeLa cells by immunofluorescence.
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Flow Cytometry-Anti-RanGAP1 antibody(TA310812); Overlay histogram showing Jurkat cells stained with TA310812 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). This antibody gave a positive signal in Jurkat cells under the same conditions.
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