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Anti-RANGAP1 Antibody EPR3295
Also for RANGAP1 (NM_002883)
|A synthetic peptide corresponding to residues in human RanGAP1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1,000 - 5,000; IHC 1:100 - 250; ICC: 1:100 - 250;
|Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.|
|Homo sapiens Ran GTPase activating protein 1 (RANGAP1), transcript variant 2|
|Fug1; RANGAP; SD|
|RanGAP1 is the activator of the Ras-related nuclear GTPase Ran, which is involved in the nucleocytoplasmic transport, mitotic spindle assembly, cell cycle control and nuclear envelope (NE) formation (1, 2). Its roles are accomplished by the asymmetric distribution of its GTP- and GDP-bound forms, enabled by the specific localization of Ran accessory proteins, the Ran GTPase-activating protein RanGAP and the nucleotide exchange factor RCC. Mammalian RanGAP1 is targeted to the NE during interphase and to the spindle and kinetochores during mitosis via SUMOylated C-terminal domain and interaction with the nucleoporin Nup358/RanBP2 (2). RanGAP1 is highly expressed in brain, thymus and testis (3).|
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Western blot - RanGAP1 antibody [EPR3295]; All lanes : Anti-RanGAP1 antibody [EPR3295] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : MCF-7 cell lysate.Lane 3 : SH-SY5Y cell lysate.Lane 4 : A549 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit antibody at 1/2000 dilution.Predicted band size : 64 kDa.Observed band size : 64 kDa.Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RanGAP1 antibody [EPR3295]; b92360 at 1/100 dilution staining RanGAP1 in paraffin-embedded (1) Human breast carcinoma tissue and (2) Human testis tissue by immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - RanGAP1 antibody [EPR3295]; ab92360 at 1/100 dilution staining RanGAP1 in HeLa cells by immunofluorescence.
Flow Cytometry-Anti-RanGAP1 antibody [EPR3295](ab92360); Overlay histogram showing Jurkat cells stained with ab92360 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.