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Anti-RANBP3 Antibody EPR5088(2)
Also for RANBP3 (NM_003624)
|A synthetic peptide corresponding to residues in human RanBP3 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:100 - 1:500
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Rat
|Homo sapiens RAN binding protein 3 (RANBP3), transcript variant RANBP3-a|
|RanBP3 is a protein with a RanBD1 domain that is found in both the nucleus and cytoplasm. It plays a role in nuclear export as part of a heteromeric complex (1). RanBP3 binds to the Crm1 transport receptor and inhibits the binding of unloaded Crm1 to the NPC. In addition, it increases the affinity of Crm1 for both Ran-GTP and nuclear export sequences. In this manner, RanBP3 coordinates efficient cargo loading and RanGTP binding (2). Moreover, RanBP3 has been shown to bind to RCC1 in a Ran-dependent manner and to increase the nucleotide exchange activity of RCC1, resulting in high concentrations of RanGTP in the vicinity of RCC1. Consequently, RanBP3 acts as a scaffold protein by which components of the export complex are concentrated around the RCC1 site, thus promoting complex assembly (3).|
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Western blot - Anti-RanBP3 antibody [EPR5088(2)]; Anti-RanBP3 antibody [EPR5088(2)] at 1/1000 dilution + 293T lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 60 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP3 antibody [EPR5088(2)]; Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling RanBP3 with ab134052 at 1/100 dilution.